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| Advisor(s) | apt. Fitri Wahyu Dhini, S.Farm., M.K.M. | |
| Author(s) | PANGARIBUAN, IMMANUEL PANDAPOTAN | |
| Issued Date | 2026 | |
| Abstract | Aloe vera is a plant with significant potential as a traditional medicine due to its content of secondary metabolites such as flavonoids, specifically quercetin. This study is aimed to identify and quantify quercetin in the ethanolic extract of Aloe vera using Thin-Layer Chromatography (TLC) and High-Performance Liquid Chromatography (HPLC) methods, as well as to validate these methods. The crude drug powder was extracted via reflux using 96% ethanol, yielding an extract recovery of 13.25%. Characterization of the crude drug revealed a moisture content of 8.12%, water-soluble extractive value of 18.88%, ethanol-soluble extractive value of 16.66%, total ash content of 3.83%, and acid-insoluble ash content of 0.5%; all parameters met the standards of the Indonesian Herbal Pharmacopoeia. Qualitative analysis using TLC yielded a positive result for quercetin with an Rf value of 0.60, close to that of the quercetin standard (0.62). Quantitative determination was performed using an HPLC system with a C18 column, a methanol:water (60:40 v/v) mobile phase, a flow rate of 1.2 mL/min, and detection at a wavelength of 370 nm. Method validation yielded a linear regression equation of y = 14193x - 388418 with an R² of 0.9994, a precision value (%RSD) of 0.69%, and accuracy (%recovery) ranging from 99.89% to 100.15%, all meeting acceptance criteria. The obtaine of HPLC method contained of LOD and LOQ values were 2.86 ppm and 8.68 ppm, respectively. Quantitative analysis showed that the ethanolic extract of the leaves contained quercetin at an average concentration of 70.81 ppm. It can be concluded that the HPLC method used is valid, selective, and sensitive for the determination of quercetin content in aloe vera. | 1 |
| Item Type | Skripsi | |
| Keywords | Ekstrak lidah buaya (aloe vera extraction), kuersetin (quercetin), metode KLT (TLC method), metode HPLC (HPLC method) | 1 |
| Collection | Fakultas Farmasi, S1-Farmasi | |
| Publisher | INSTITUT KESEHATAN DELI HUSADA | |
| Submitted Date | 29-08-2026 | |
| Updated Date | 29-08-2026 | |
| URI | https://repo.delihusada.mediantarakreasindo.com/handle/123456789/10963 | |
| File |
2218085_ImmanuelPandapotanPangaribuan_Skripsi_S1.pdf
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