PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK ETANOL, FRAKSI N-HEKSAN DAN FRAKSI ETIL ASETAT PADA KULIT BUAH MARKISA UNGU (Passiflora edulis sims) DENGAN METODE SPEKTROFOTOMETRI UV-Vis

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Advisor(s) apt. Masria Phetheresia Sianipar, S.Farm.,M.Si.
Author(s) LOLO SIHOMBING
Issued Date 2026
Abstract The peel of the purple passion fruit (Passiflora edulis Sims) contains flavonoid compounds with potential antioxidant activity. This study aimed to determine the total flavonoid content in the ethanol extract, n-hexane fraction, and ethyl acetate fraction of purple passion fruit peel using UV-Vis spectrophotometry and to validate the method employed. Quantification was performed using aluminum chloride (AlCl₃) and sodium acetate reagents, with quercetin as the reference standard, at a wavelength of 432 nm. The results showed total flavonoid contents of 10.71% for the n-hexane fraction, 14.52% for the ethanol extract, and 22.43% for the ethyl acetate fraction. The ethyl acetate fraction exhibited the highest total flavonoid content, indicating a greater distribution of flavonoids in the semi-polar fraction. Method validation results met all requirements; thus, the UV-Vis spectrophotometric method was deemed valid and suitable for determining total flavonoid content in purple passion fruit peel. Further research using HPLC is recommended to achieve more specific results and greater accuracy in flavonoid determination. 1
Item Type Skripsi
Keywords Passiflora Edulis Sims, Kulit (Purple), Kulit Buah Markisa Ungu, Flavonoid Total, Fraksi Etil Asetat, Spektrofotometri UV-Vis 1
Collection Fakultas Farmasi, S1-Farmasi
Publisher INSTITUT KESEHATAN DELI HUSADA
Submitted Date 09-09-2026
Updated Date 19-09-2026
URI No URI
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